MSH6 Recombinant Mouse Monoclonal Antibody [A4C4-R]

MSH6 Recombinant Mouse Monoclonal Antibody [A4C4-R]

• Description: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.
• Product Name Alternative: DNA mismatch repair protein Msh6 antibody G/T mismatch binding protein antibody G/T mismatch-binding protein antibody GTBP antibody GTMBP antibody hMSH6 antibody HNPCC 5 antibody HNPCC5 antibody HSAP antibody MSH 6 antibody MSH6 antibody MSH6_HUMAN antibody mutS (E. coli) homolog 6 antibody MutS alpha 160 kDa subunit antibody MutS homolog 6 (E. coli) antibody mutS homolog 6 antibody MutS-alpha 160 kDa subunit antibody p160 antibody Sperm associated protein antibody
• Abbreviation: DNA mismatch repair protein Msh6 hMSH6 G/T mismatch-binding protein MutS protein homolog 6 MutS-alpha 160 kDa subunit (p160 MSH6 GTBP
• CAS Number: 9000-83-3
• Swiss Prot: P52701 Human, P54276 Mouse
• Cellular Locus: Nucleus, Chromosome.
• Host: Mouse
• Species Reactivity: Human, Mouse
• Immunogen: Synthetic peptide corresponding to C-terminal Human MSH6.
• Isotype: IgG1
• Clone: A4C4-R
• Conjugation: Non-conjugated
• Type: Recombinant Mouse monoclonal Antibody
• Applications: WB, IHC-P
• Positive Control: SH-SY5Y cell lysate, 293T cell lysate, NIH/3T3 cell lysate, human colon carcinoma tissue, human stomach carcinoma tissue, mouse testis tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000 ;IHC-P: 1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 152.8 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes