MSH6 Recombinant Rabbit Monoclonal Antibody [PD00-26]

MSH6 Recombinant Rabbit Monoclonal Antibody [PD00-26]

• Description: This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. Tonsil is found to be a recommendable positive tissue control for MSH6. Virtually all mantle zone B-cells must show an at least weak to moderate nuclear staining reaction, while a moderate to strong nuclear staining reaction must be seen in the proliferating germinal centre B-cells. Colon adenocarcinoma with loss of MSH6 expression is recommended as negative tissue control. No nuclear staining reaction should be seen in the neoplastic cells, whereas a nuclear staining reaction must be seen in stromal cells serving as internal positive tissue control.
• Product Name Alternative: DNA mismatch repair protein Msh6 antibody G/T mismatch binding protein antibody G/T mismatch-binding protein antibody GTBP antibody GTMBP antibody hMSH6 antibody HNPCC 5 antibody HNPCC5 antibody HSAP antibody MSH 6 antibody MSH6 antibody MSH6_HUMAN antibody mutS (E. coli) homolog 6 antibody MutS alpha 160 kDa subunit antibody MutS homolog 6 (E. coli) antibody mutS homolog 6 antibody MutS-alpha 160 kDa subunit antibody p160 antibody Sperm associated protein antibody
• Abbreviation: MSH6
• CAS Number: 9000-83-3
• Swiss Prot: P52701 Human, P54276 Mouse
• Cellular Locus: Nucleus, Chromosome.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide within Human MSH6 aa 350-450 (internal sequence).
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P, IF-Cell, IF-Tissue
• Positive Control: A549 cell lysate, HepG2 cell lysate, human tonsil tissue, human colon carcinoma tissue, human stomach carcinoma tissue, mouse small intestine tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000 ;IHC-P: 1:1,000 ;IF-Cell: 1:100 ;IF-Tissue: 1:200
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 153 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes