Phospho-PKC alpha (T638) Recombinant Rabbit Monoclonal Antibody [JF0964]

Phospho-PKC alpha (T638) Recombinant Rabbit Monoclonal Antibody [JF0964]

• Description: Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor-promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (α, βI, βII, γ, δ, ε, ζ, η, θ, λ/ι, μ and ν). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca²⁺. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.
• Product Name Alternative: AAG6 antibody Aging associated gene 6 antibody aPKC antibody KPCA_HUMAN antibody PKC alpha antibody PKC-A antibody PKC-alpha antibody PKCA antibody PRKACA antibody PRKCA antibody Protein Kinase C alpha antibody Protein kinase C alpha type antibody
• Abbreviation: PKC-A, PKC-alpha, PRKCA
• CAS Number: 9000-83-3
• Swiss Prot: P17252 Human, P20444 Mouse, P05696 Rat
• Cellular Locus: Cytoplasm, Cell membrane, Mitochondrion membrane, Nucleus.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr638 of Human PKC alpha aa 622-667 / 672.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP
• Positive Control: HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, HeLa, NIH/3T3, C6, human breast cancer tissue, rat brain tissue, mouse brain tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500-1:5,000 ;IF-Cell: 1:50-1:500 ;IF-Tissue: 1:50-1:500 ;IHC-P: 1:50-1:1,000 ;IP: Use at an assay dependent concentration.
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 77 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes