Phospho-PKC (S729) Recombinant Rabbit Monoclonal Antibody [JE77-54]

Phospho-PKC (S729) Recombinant Rabbit Monoclonal Antibody [JE77-54]

• Description: In cell biology, Protein kinase C, commonly abbreviated to PKC (EC 2.7.11.13), is a family of protein kinase enzymes that are involved in controlling the function of other proteins through the phosphorylation of hydroxyl groups of serine and threonine amino acid residues on these proteins, or a member of this family. PKC enzymes in turn are activated by signals such as increases in the concentration of diacylglycerol (DAG) or calcium ions (Ca2+). Hence PKC enzymes play important roles in several signal transduction cascades. In biochemistry, the PKC family consists of fifteen isozymes in humans. They are divided into three subfamilies, based on their second messenger requirements: conventional (or classical), novel, and atypical. Conventional (c)PKCs contain the isoforms α, βI, βII, and γ. These require Ca2+, DAG, and a phospholipid such as phosphatidylserine for activation. Novel (n)PKCs include the δ, ε, η, and θ isoforms, and require DAG, but do not require Ca2+ for activation. Thus, conventional and novel PKCs are activated through the same signal transduction pathway as phospholipase C. On the other hand, atypical (a)PKCs (including protein kinase Mζ and ι / λ isoforms) require neither Ca2+ nor diacylglycerol for activation.
• Product Name Alternative: KPCA_HUMAN antibody PKC alpha antibody PKC beta antibody PKC delta antibody PKC epsilon antibody PKC gamma antibody PKC zeta antibody PKC-A antibody PKC-alpha antibody PKC2 antibody PKCA antibody PKCB antibody PKCD antibody PKCE antibody PKCG antibody PRKCA antibody PRKCB antibody PRKCB1 antibody PRKCB2 antibody PRKCD antibody PRKCE antibody PRKCG antibody PRKCZ antibody Protein kinase C alpha antibody Protein kinase C alpha type antibody Protein kinase C antibody Protein kinase C beta antibody Protein kinase C delta antibody Protein kinase C epsilon antibody Protein kinase C gamma antibody Protein kinase C zeta antibody
• Abbreviation: Protein kinase C epsilon type EC:2.7.11.13 nPKC-epsilon
• CAS Number: 9000-83-3
• Swiss Prot: P05129 Human, P05771 Human, P17252 Human, Q02156 Human, Q05513 Human, Q05655 Human
• Cellular Locus: Cytoplasm, cytoskeleton, Cell membrane, perinuclear region, Nucleus.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding S729 of human PKC.
• Isotype: IgG
• Clone: JE77-54
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB
• Positive Control: HeLa cell lysate, HeLa treated with 200nM TPA for 30 minutes cell lysate, C2C12 cell lysate, C2C12 treated with 200nM TPA for 30 minutes cell lysate, C6 cell lysate, C6 treated with 200nM TPA for 10 minutes cell lysate.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 84 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes