Anti-Phospho-IKB alpha (S32) Antibody [ST53-05]

Anti-Phospho-IKB alpha (S32) Antibody [ST53-05]

• Description: On the basis of both functional and structural considerations, members of the IκB family of proteins can be divided into four groups. The first of these groups, IκB-α, includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFkB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to κB sites, suggesting that the IκB-α family binds to the p65 subunit of p50-p65 heterocomplex through ankyrin repeats. The second member of the IκB family is represented by a protein designated IκB-β. The third group of IκB proteins is represented by IκB-γ, which is identical in sequence with the C-terminal domain of the p110 precursor of NFkB p50 and is expressed predominantly in lymphoid cells. An additional IκB family member, IκB-ε, has several phosphorylated forms and is primarily found complexed with Rel A and/or c-Rel.
• Product Name Alternative: I kappa B alpha antibody I-kappa-B-alpha antibody IkappaBalpha antibody IkB-alpha antibody IKBA antibody IKBA_HUMAN antibody IKBalpha antibody MAD 3 antibody MAD3 antibody Major histocompatibility complex enhancer-binding protein MAD3 antibody NF kappa B inhibitor alpha antibody NF-kappa-B inhibitor alpha antibody NFKBI antibody NFKBIA antibody Nuclear factor of kappa light chain gene enhancer in B cells antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody
• Abbreviation: IkB-alpha, IkappaBalpha, NFKBIA, IKBA, MAD3, NFKBI
• CAS Number: 9007-83-4
• Swiss Prot: P25963 Human; Q9Z1E3 Mouse
• Cellular Locus: Cytoplasm, Nucleus.
• Host: Rabbit
• Species Reactivity: Human, Mouse
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser32 of human IKB alpha.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IP
• Positive Control: SK-Br-3 cell lysates, Hela,NIH-3T3 cell lysates.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IP: Use at an assay dependent concentration.
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: 36 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: True