Histone H2A.Z Recombinant Rabbit Monoclonal Antibody [ST46-09]

Histone H2A.Z Recombinant Rabbit Monoclonal Antibody [ST46-09]

• Description: Histone H2A.Z is a protein that in humans is encoded by the H2AFZ gene. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The H2AFZ gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. Histone H2AZ is a variant of histone H2A, and is used to mediate the thermosensory response, and is essential to perceive the ambient temperature. Nucleosome occupancy of H2A.Z decreases with temperature, and in vitro assays show that H2A.Z-containing nucleosomes wrap DNA more tightly than canonical H2A nucleosomes in Arabidopsis. Positioning of H2A.Z containing nucleosomes around transcription start sites has now been shown to affect the downstream gene expression.
• Product Name Alternative: Histone H2A.Z H2A/z H2az1 H2afz H2az
• Abbreviation: HIST1H2AB
• CAS Number: 9000-83-3
• Swiss Prot: P0C0S5 Human, P0C0S6 Mouse, P0C0S7 Rat
• Cellular Locus: Nucleus, Chromosome, Nucleosome core.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide within human Histone H2A.Z aa 1-50.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P, IF-Cell, IF-Tissue, ChIP
• Positive Control: Hela cells, Human spleen tissue, mouse colon tissue, human lung carcinoma tissue, human kidney tissue, human liver carcinoma tissue, human liver tissue, human thyroid tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500-1:1,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 14 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes