Cdk4 Recombinant Rabbit Monoclonal Antibody [SD20-42]

Cdk4 Recombinant Rabbit Monoclonal Antibody [SD20-42]

• Description: Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression . Another family of proteins, Cdk inhibitors, also plays a role in regulating the cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1-PCTAIRE-3, PITALRE and PITSLRE. Cdk4, in complex with D-type cyclins, is thought to regulate cell growth during the G1 phase of the cell cycle. This association with a D-type cyclin upregulates Cdk4 activity, whereas binding to the Cdk inhibitor p16 downregulates Cdk4 activity. Activation of the Cdk4-cyclin complexes requires phosphorylation on a single threonyl residue of Cdk4, catalyzed by a Cdk-activating protein (CAK).
• Product Name Alternative: Cdk 4 antibody cdk4 antibody CDK4 protein antibody CDK4_HUMAN antibody Cell division kinase 4 antibody Cell division protein kinase 4 antibody CMM 3 antibody CMM3 antibody Crk3 antibody Cyclin dependent kinase 4 antibody Cyclin-dependent kinase 4 antibody Melanoma cutaneous malignant 3 antibody MGC14458 antibody p34 cdk4 antibody PSK J3 antibody PSK-J3 antibody
• Abbreviation: CDK4
• CAS Number: 9000-83-3
• Swiss Prot: P11802 Human, P30285 Mouse, P35426 Rat
• Cellular Locus: Nucleus, Nucleus membrane, Cytoplasm.
• Host: Rabbit
• Species Reactivity: Human
• Immunogen: Synthetic peptide within C-terminal human Cdk4.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
• Positive Control: Hela cell lysate, MCF-7 cell lysate, Hela, MCF-7, NIH/3T3, human breast carcinoma tissue, human stomach carcinoma tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000-1:5,000;IF-Cell: 1:100-1:500;IF-Tissue: 1:100-1:500;IHC-P: 1:50-1:200;FC: 1:50-1:100
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: 34 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes