NOX2/gp91phox Recombinant Rabbit Monoclonal Antibody [SN07-16]

NOX2/gp91phox Recombinant Rabbit Monoclonal Antibody [SN07-16]

• Description: Mox1 and the glycoprotein gp91-phox are largely related proteins that are essential components of the NADPH oxidase. The superoxide-generating NADPH oxidase is present in phagocytes, neuroepithelial bodies, vascular smooth muscle cells and endothelial cells. It includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane, where they associate with the flavocytochrome cytochrome b558 to form the active enzyme complex. The p22- and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Mox1 and gp91 contain identical C-terminal sequence identity, yet they have distinct expression patterns. gp91-phox is expressed in eosinophils, neutro-phils, monocytes and B-lymphocytes, whereas Mox1 is predominantly detected in the colon, and low expression is also detected in the uterus and prostate. Mox1 is also upregulated in vascular smooth-muscle cells in response to PDGF stimulation, which collectively indicates that Mox1 may function analogously to gp91-phox, yet regulate the NADPH superoxide production in non-phagocytic cells.
• Product Name Alternative: AMCBX2 antibody CGD antibody CGD91-phox antibody CY24B_HUMAN antibody CYBB antibody Cytochrome b 245, beta polypeptide antibody Cytochrome b (558) beta chain antibody Cytochrome b (558) subunit beta antibody Cytochrome b-245 heavy chain antibody Cytochrome b558 subunit beta antibody GP91 PHOX antibody gp91-1 antibody gp91-phox antibody GP91PHOX antibody Heme-binding membrane glycoprotein gp91phox antibody NADPH oxidase 2 antibody Neutrophil cytochrome b 91 kDa polypeptide antibody NOX2 antibody p22 phagocyte B-cytochrome antibody P91 PHOX antibody p91-PHOX antibody Superoxide-generating NADPH oxidase heavy chain subunit antibody
• Abbreviation: Cytochrome b558 subunit beta, CYBB
• CAS Number: 9000-83-3
• Swiss Prot: P04839 Human, Q61093 Mouse,
• Cellular Locus: Cell membrane.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide within Human NOX2 aa 131-180 / 570.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB
• Positive Control: HepG2 cell lysate, MCF7 cell lysate, mouse brain tissue lysate, mouse spleen tissue lysate, rat brain tissue lysate, rat spleen tissue lysate.
• Concentration: 1 mg/mL
• Dilution: WB: 1:2,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 65 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes