nNOS Recombinant Rabbit Monoclonal Antibody [ST520]

nNOS Recombinant Rabbit Monoclonal Antibody [ST520]

• Description: Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types.
• Product Name Alternative: 2310005C01Rik antibody BNOS antibody Constitutive NOS antibody EC 1.14.13.39 antibody IHPS 1 antibody IHPS1 antibody N-NOS antibody NC-NOS antibody neuronal Nitric Oxide Synthase antibody Neuronal NOS antibody Nitric oxide synthase , neuronal, included antibody Nitric oxide synthase 1 (neuronal) antibody Nitric oxide synthase 1 antibody Nitric oxide synthase, brain antibody Nitric oxide synthase, penile neuronal, included antibody NNOS antibody NO antibody NOS 1 antibody NOS antibody NOS type I antibody NOS-I antibody NOS1 antibody NOS1_HUMAN antibody Peptidyl-cysteine S-nitrosylase NOS1 antibody
• Abbreviation: N-NOS, nNOS, NOS1
• CAS Number: 9000-83-3
• Swiss Prot: P29475 Human, Q9Z0J4 Mouse, P29476 Rat
• Cellular Locus: Cell membrane, sarcolemma, Cell projection, dendritic spine.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide within Human nNOS aa 1398-1434 / 1434.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Tissue, IHC-P, IP
• Positive Control: Mouse brain tissue lysate, Mouse hippocampus tissue lysate, Mouse cerebellum tissue lysate, Rat hippocampus tissue lysate, Rat cerebellum tissue lysate, human skeletal muscle tissue, rat brain tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000-1:2,000 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200-1:1,000 ;IP: Use at an assay dependent concentration.
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 161 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes