Recombinant Mouse Interleukin-2/IL-2 Protein (active)

• Background:

  Interleukin-2 (IL-2) is an interleukin; a type of cytokine signaling molecule in the immune system; belongs to the IL-2 family. It is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells; NK cells; lymphokine-activated killer cells; monocytes; macrophages and oligodendrocytes.

• Abbreviation:

  Interleukin-2/IL-2

• Synonyms:

  Interleukin-2; IL-2; T-Cell Growth Factor; TCGF; Aldesleukin

• UniProt:

  P04351

• Accession Number:

  P04351

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  Ala21-Gln169

• Field of Research:

  Immunology

• Endotoxin:

  ≤ 0.1 EU per μg by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured in a cell proliferation assay using HT-2 mouse T cells. The ED50 for this effect is 0.88-3.5 ng/mL, corresponding to a specific activity of 2.86x105-1.14x106 units/mg.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  18.07 kDa

• Observed Molecular Weight:

  22 - 25 kDa

• Formulation:

  Lyophilized from a 0.22 µm filtered solution of PBS, pH 7.4.

• Species:

  Mouse