PUMA Antibody (alpha/beta) / BBC3

• Description:

  Bcl-2-binding component 3, isoforms 1/2 (BBC3), also called p53 upregulated modulator of apoptosis (PUMA), is a pro-apoptotic member of the Bcl-2 protein family. The gene is located at 19q. The transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91% amino acid identity with the murine sequence. Bcl-2 family members can form hetero- or homodimers, and they act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and the protein has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes two BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation. Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.

• UniProt:

  Q9BXH1

• Host:

  Rabbit

• Immunogen:

  Amino acids EQWAREIGAQLRRMADDLNAQYE were used as the immunogen for the PUMA antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Format:

  Antigen affinity purified

• Buffer:

  0.5mg/ml if reconstituted with 0.2ml sterile DI water

• Reconstitution:

  After reconstitution, the PUMA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This PUMA antibody is available for research use only.

• CAS Number:

  9007-83-4