Spectrin Antibody (alpha and beta)

• Description:

  Spectrin, the predominant component of the membrane skeleton of the red cell, is essential in determining the properties of the membrane including its shape and deformability. It consists of 2 nonidentical subunits, alpha and beta. Spectrin is present in the red cell membrane in a tetrameric or possibly higher polymeric form through head-to-head self-association of heterodimers that are linked by actin polymers and protein 4.1 to form a 2-dimensional network. Non-erythroid spectrin gene is mapped to human chromosome 2. Spectrin mutations cause spinocerebellar ataxia type 5.

• Specifications:

  Western blot: 2-4 µg/mL

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Human erythrocyte spectrin was used as the immunogen for this Spectrin antibody (alpha and beta) .

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  SB-SP1

• Applications:

  WB

• Purity:

  Ascites

• Format:

  Ascites

• Buffer:

  0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

• Reconstitution:

  After reconstitution, the Spectrin antibody (alpha and beta) can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Spectrin antibody (alpha and beta) is available for research use only.

• Storage Conditions:

  After reconstitution, the Spectrin antibody (alpha and beta) can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the Spectrin antibody (alpha and beta) may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4