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PUMA Antibody

CAT: 0800-R30371Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30371Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The p53 upregulated modulator of apoptosis, or PUMA, is a pro-apoptotic member of the Bcl-2 protein family. The PUMA gene is located at 19q. PUMA transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91% amino acid identity with the murine sequence. Bcl-2 family members can form hetero- or homodimers, and they act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and PUMA has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes 2 BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation. Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
Q9BXH1
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the C-terminus of human PUMA (ADDLNAQYERRRQEE) was used as the immunogen for this PUMA antibody (100% homologous in human, mouse and rat) . This sequence is common to isoforms alpha, beta, delta and gamma.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the PUMA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PUMA antibody is available for research use only.
Storage Conditions
After reconstitution, the PUMA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the PUMA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of PUMA antibody and Lane 1: HeLa; 2: rat kidney; Predicted molecular weight: 21/14/10/26kDa (isoforms alpha/beta/delta/gamma) .
Product Datasheet
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