CD44 Antibody

• Description :

  The CD44 gene, which is a transmembrane protein, is expressed as a family of molecular isoforms generated from alternative RNA splicing and posttranslational modifications. The gene, which contains 19 exons spanning some 50 kb of genomic DNA, is a widely expressed integral membrane protein that acts as a receptor for hyaluronan (HA) and is important to cell-extracellular matrix interaction. CD44 binding with HA can play an important role in cellular aggregation and tumor cell growth. CD44 is necessary for limb development and functions in a novel growth factor presentation mechanism. A specific CD44 splice variant is crucial for the proliferation of these mesenchymal cells. CD44 glycoproteins are involved in leukocyte extravasation but also in the regulation of growth factor activation, stability, and signaling. Moreover, it plays a pivotal role in arteriogenesis.

• Specifications :

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells

• UniProt :

  P16070

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  An amino acid sequence from the C-terminus of human CD44 (DETRNLQNVDMKIGV) was used as the immunogen for this CD44 antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB, IHC-P, FACS

• Purity :

  Antigen affinity

• Format :

  Purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution :

  After reconstitution, the CD44 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This CD44 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the CD44 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  The stated application concentrations are suggested starting amounts. Titration of the CD44 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Location :

  Cell surface, cytoplasmic

• Image Legend :

  IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with CD44 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.