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HCAM Antibody / CD44

CAT: 0800-V2068IHC-7MLSize: 7 mLDry Ice: NoHazardous: No
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CAT#:0800-V2068IHC-7MLSize:7 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes a cell surface glycoprotein of 80-95kDa, called CD44, or HCAM, on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V) . Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44-s) . Higher molecular weight isoforms are described in epithelial cells (CD44-v), which are believed to function in intercellular adhesion and stromal binding. HCAM antibody immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.
CAS Number
9007-83-4
Specifications
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Host
Mouse
Reactivity
Human
Immunogen
Stimulated human leukocytes were used as the immunogen for this CD44 / HCAM antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
156-3C11
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the HCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This HCAM antibody is available for research use only.
Storage Conditions
Store the HCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Location
Cell surface, cytoplasmic
Image Legend
IHC testing of FFPE human tonsil stained with HCAM antibody (clone 156-3C11) .

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