CD45 Antibody

• Description:

  CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out) . The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P08575

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human PTPRC protein was used as the immunogen for the CD45 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  PTPRC/6780

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Aliquot the CD45 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CD45 antibody is available for research use only.

• Storage Conditions:

  Aliquot the CD45 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the CD45 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, Cytoplasm

• Image Legend:

  IHC staining of FFPE human tonsil tissue with CD45 antibody (clone PTPRC/6780) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.