ACTA2 Antibody

• Description:

  Actin is a major component of the cytoskeleton and is present in most cell types.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunohistochemistry (Frozen) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL

• UniProt:

  P62736

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Amino acids 1-10 of alpha-smooth muscle actin was used as the immunogen for this ACTA2 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a

• Clone:

  1A4

• Applications:

  WB, IHC-P, IHC-F, IF

• Purity:

  Unpurified ascites

• Format:

  Ascites

• Buffer:

  Mouse ascites fluid with 2.5% BSA and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the ACTA2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This ACTA2 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the ACTA2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the ACTA2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Immunofluorescent staining of FFPE human placental tissue with ACTA2 antibody (green), CD31 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA buffer for 20 min.