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HNF4A Antibody

CAT: 0800-RQ6999Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6999Size:100 µg
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Description
Hepatocyte nuclear factor 4 alpha (HNF4A), also known as NR2A1, is a nuclear receptor that in humans is encoded by the HNF4A gene. It is mapped to 20q13.12. HNF4A is a nuclear transcription factor that binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene plays a role in development of the liver, kidney, and intestines. HNF4A is required for the PXR and CAR-mediated transcriptional activation of CYP3A4. This gene also plays a pivotal role in the expression and synthesis of SHBG, an important glycoprotein made primarily in the liver, which in addition to lowering insulin-resistance also serves in reducing levels of free Oestrogen as-well as prolonging the half-life of Testosterone.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL
UniProt
P41235
Host
Mouse
Reactivity
Human, Rat
Immunogen
Recombinant human protein (amino acids Q164-I474) was used as the immunogen for the HNF4A antibody.
Clonality
Monoclonal
Isotype
IgG2a
Clone
6C8E9
Applications
WB, IHC-P
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the HNF4A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This HNF4A antibody is available for research use only.
Storage Conditions
After reconstitution, the HNF4A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the HNF4A antibody should be determined by the researcher.
Location
Predominantly nuclear, present in cytoplasm in cells with high HNF4A expression
Image Legend
IHC staining of FFPE rat colon tissue with HNF4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

UniProtKB · P41235

Hepatocyte nuclear factor 4-alpha

HNF4A_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P41235
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
HNF4A
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
HNF-4-alpha
EC number
—
Processing
—
Secondary accessions
A5JW41, B2RPP8, O00659, O00723, Q14540, Q5QPB8, Q6B4V5, Q6B4V6, Q6B4V7, Q92653, Q92654, Q92655, Q99864, Q9NQH0
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondPhosphoproteinUbl conjugation

Molecular function

ActivatorDNA-bindingReceptorRepressor

Coding sequence diversity

Alternative promoter usageAlternative splicing

Biological process

Biological rhythmsTranscriptionTranscription regulation

Disease

Diabetes mellitusDisease variant

Ligand

Metal-bindingZinc

Cellular component

Nucleus

Domain

Zinc-finger

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