Human Tumor Necrosis Factor-beta Recombinant

CAT: 0421-90245-BSize: 20 µgDry Ice: NoHazardous: No

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CAT#:0421-90245-BSize:20 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Background

TNF-β is a potent mediator of inflammatory and immune responses. It belongs to the TNF family of ligands and signals through TNFR1 and TNFR2. TNF-β is produced by activated T and B lymphocytes, and has similar activities to TNF-alpha. Like TNF-α, TNF-β is involved in the regulation of various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNF-β is secreted as a soluble polypeptide, but can form heterotrimers with lymphotoxin-β, which effectively anchors the TNF- β to the cell surface. TNF-β is cytotoxic to a wide range of tumor cells.

Description

Recombinant TNF-beta is a non disulfide-linked homotrimer protein, each consisting of 172 amino acids and migrates as an approximately 21 kDa protein under reducing conditions. Optimized DNA sequence encoding Human Tumor Necrosis Factor-beta chain was expressed inE. coli.

UniProt

P01375

Endotoxin

< 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Purity

≥ 95% by SDS-PAGE and HPLC

Format

Lyophilized protein

Reconstitution

Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

Shipping Conditions

Gel Packs or Dry Ice

Storage Conditions

The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

Calculated Molecular Weight

21 kDa

Formulation

Lyophilized from a 0.2 µm filtered PBS solution, pH 7.5.

UniProtKB · P01375

Tumor necrosis factor

TNFA_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P01375
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: TNF
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: O43647, Q9P1Q2, Q9UIV3

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Cellular component

Cell membraneMembraneSecreted

Molecular function

Cytokine

PTM

Disulfide bondGlycoproteinLipoproteinMyristatePhosphoprotein

Domain

Signal-anchorTransmembraneTransmembrane helix

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Alternative Products

Catalog	Name
6214	Tumor Necrosis Factor beta
22060505-1	Human Tumor Necrosis Factor-beta (Recombinant)
MBS2030088-01	Recombinant Tumor Necrosis Factor Beta (TNFb)
MBS553181-01	Human Tumor Necrosis Factor-beta Recombinant
7-01659	Recombinant Human Tumor Necrosis Factor-beta
50156P-20	Recombinant Human Tumor Necrosis Factor beta