Human Tumor Necrosis Factor-alpha Recombinant

CAT: 0421-90244-ASize: 10 µgDry Ice: NoHazardous: No

1 / 1

CAT#:0421-90244-ASize:10 µg

Selected

24/48H Stock Items & 2 to 6 Weeks non Stock Items.

Quick Request Actions

Background

Tumor Necrosis Factor is secreted by macrophages, monocytes, neutrophils, T-cells, and NK- cells following their stimulation by bacterial lipopolysaccharides. Cells expressing CD4 secrete TNF-alpha while CD8 (+) cells secrete little or no TNF-alpha. Stimulated peripheral neutrophilic granulocytes but also unstimulated cells and also a number of transformed cell lines, astrocytes, microglial cells, smooth muscle cells, and fibroblasts also secrete TNF. Human milk also contains this factor. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL-2, GM-CSF, SP, Bradykinin, Immune complexes, inhibitors of cyclooxygenase and PAF (platelet activating factor) . Human TNF-alpha is a non-glycosylated protein of 17 kDa and a length of 157 amino acids. Murine TNF-alpha is N-glycosylated. Homology with TNF-beta is approximately 30%. TNF-alpha forms dimers and trimers.

Description

Recombinant TNF-alpha is a disulfide-linked monomer protein, consisting of 158 amino acids and migrates as an approximately 17 kDa protein under reducing conditions. Optimized DNA sequence encoding Human Tumor Necrosis Factor-alpha chain was expressed inE. coli.

UniProt

P01375

Endotoxin

Endotoxin level was found to be < 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Purity

≥ 95% by SDS-PAGE and HPLC

Format

Lyophilized protein

Reconstitution

A quick mix of the vial followed by reconstitution in distilled water to concentration not less than 0.1 mg/ml. This solution can then be diluted into other buffers.

Shipping Conditions

Gel Packs or Dry Ice

Storage Conditions

The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

Calculated Molecular Weight

17 kDa

Formulation

Lyophilized from a 0.2 µm filtered concentrated (1 mg/ml) solution in PBS, pH 7.2.

UniProtKB · P01375

Tumor necrosis factor

TNFA_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P01375
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: TNF
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: O43647, Q9P1Q2, Q9UIV3

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Cellular component

Cell membraneMembraneSecreted

Molecular function

Cytokine

PTM

Disulfide bondGlycoproteinLipoproteinMyristatePhosphoprotein

Domain

Signal-anchorTransmembraneTransmembrane helix

Related Products

Catalog	Name
90246-A	Mouse Tumor Necrosis Factor-alpha Recombinant
90246-B	Mouse Tumor Necrosis Factor-alpha Recombinant
90245-A	Human Tumor Necrosis Factor-beta Recombinant
90245-B	Human Tumor Necrosis Factor-beta Recombinant
90157-B	Human Hepatocyte Growth Factor alpha chain Recombinant
90157-A	Human Hepatocyte Growth Factor alpha chain Recombinant
90237-A	Human Stromal Cell-Derived Factor-1 alpha Recombinant
90237-B	Human Stromal Cell-Derived Factor-1 alpha Recombinant
90257-A	Human Erythropoietin-alpha Recombinant
90257-B	Human Erythropoietin-alpha Recombinant

Alternative Products

Catalog	Name
550453	Tumor Necrosis Factor Alpha
GWB-16A8F0	TUMOR NECROSIS FACTOR-alpha
IMSTNFAR25UG	Mouse Tumor Necrosis Factor Alpha Recombinant
GWB-903A93	Tumor Necrosis Factor- Alpha Human Recombinant
22060503-1	Human Tumor Necrosis Factor-alpha (Recombinant)
7-01637	Recombinant Human Tumor Necrosis Factor-alpha
7-01653	Recombinant Rabbit Tumor Necrosis Factor-alpha
22060637-1	Porcine Tumor Necrosis Factor-alpha (Recombinant)
50154P-50	Recombinant Human Tumor Necrosis Factor alpha
MBS2030878-01	Recombinant Tumor Necrosis Factor Alpha (TNFa)