Human Stromal Cell-Derived Factor-1 beta Recombinant

CAT: 0421-90238-ASize: 2 µgDry Ice: NoHazardous: No

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CAT#:0421-90238-ASize:2 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Background

SDF-1alpha and SDF-1beta are small cytokines belonging to the CXC- Chemokines. SDF-1 is identical with a chemokine reported to function as a pre-B-cell growth factor in the presence of IL-7 and isolated originally from a murine bone marrow stromal cell line. Human SDF-1alpha and SDF-1beta are encoded by a single gene and arise by alternative splicing. SDF-1 acts on lymphocytes and monocytes but not neutrophilsin vitroand is a highly potent chemoattractant for mononuclear cellsin vivo. In addition, SDF-1 also induces intracellular actin polymerization in lymphocytes. SDF acts as a chemoattractant for human hematopoietic progenitor cells expressing CD34 giving rise to mixed types of progenitors, and more primitive types. The chemotactic response is inhibited by pertussis toxin. Chemotaxis of CD34 (+) cells in response to SDF is increased by IL-3in vitro. SDF has been shown also to induce a transient elevation of cytoplasmic calcium in these cells.

Description

Recombinant human stromal cell-derived factor-1β (SDF1β), also known as CXCL12 or Pre-B cell growth-stimulating factor (PBSF), is a disulfide-linked monomeric protein consisting of 73 a.a. and migrates as an approximately 8 kDa protein under reducing and non-reducing conditions. Optimized DNA sequence encoding human SDF1β mature chain was expressed inE. coli.

UniProt

P48061

Endotoxin

< 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Purity

≥ 97% by SDS-PAGE and HPLC

Format

Lyophilized protein

Reconstitution

Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

Shipping Conditions

Gel Packs or Dry Ice

Storage Conditions

The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

Calculated Molecular Weight

8 kDa

Formulation

Lyophilized from a 0.2 µm filtered solution (1 mg/ml) containing 20 mM phosphate buffer, pH 7.0, and 150 mM NaCl.

UniProtKB · P48061

Stromal cell-derived factor 1

SDF1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P48061
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: CXCL12
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: SDF-1, hSDF-1
EC number: —
Processing: Precursor
Secondary accessions: B2R4G0, E7EVL0, H7BYN8, Q2L985, Q2L986, Q2L988, Q5IT36, Q6ICW0, Q9H554

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

ChemotaxisHost-virus interaction

Molecular function

CytokineGrowth factor

PTM

Disulfide bond

Cellular component

Secreted

Domain

Signal

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