Tumor Necrosis Factor beta

Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Dry Ice Shipment: No

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CAT:

246-6214

Size:

20 µg

Price:

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Tumor Necrosis Factor beta

Description: Tumor Necrosis Factor – Beta (TNF-β), also known as lymphotoxin-alpha is a human recombinant protein that can be used in a variety of applications including cytotoxicity, cell proliferation assays, and apoptosis. This product is best used for inducing apoptosis to create a positive control.
Assay Principle: 1. To make a 5 mg/mL DAPI stock solution (14.3 mM), dissolve the contents of one vial (10 mg) in 2 mL of deionized water or dimethylformamide (DMF). Note: DAPI is not very soluble in phosphate-buffered saline (PBS)., 2. For long term storage, the stock solution can be aliquoted and stored at -20°C. For short term storage, the solution can be kept at 2-6°C, protected from light. When handled properly, DAPI solutions are stable for at least six months., 3. Dilute the DAPI stock solution to 3 µM in staining buffer. A 1 mL volume will be required for each cell sample., 4. Centrifuge the cell suspension and discard the supernatant. Tap to loosen the pellet and add 1 mL of DAPI diluted in staining buffer., 5. Incubate for 15 minutes at room temperature., 6. Analyze by flow cytometry in the presence of the dye. If the cells are to be viewed by fluorescence microscopy, centrifuge the sample, remove the supernatant and resuspend cells in fresh buffer. Apply a drop of the suspension to a microscope slide, cover with a coverslip and view., For sample preparation protocols for adherent cells and suspension cells, please see the DAPI product datasheet.
Concentration: 2X concentrate

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Each item in our collection, is tested for precision and quality, ensuring dependable solutions for your research. As we continue to incorporate the latest advancements, Gentaur ensures that our product range remains relevant and versatile, providing you with the tools needed for both current and future projects.

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