Human Fibroblast Growth Factor 10 Recombinant

• Background:

  FGF-10 is involved in the initial budding as well as the continuous outgrowth of vertebrate limbs, FGF-10 mRNA is expressed preferentially in neurons but not in glial cells and may have a distinct role in the brain. Human FGF-10 is mitogenic for fetal rat keratinizing epidermal cells but not for NIH 3T3 cells. Recombinant FGF-10 induces the proliferation of human urothelial cellsin vitroand induces the proliferation of transitional epithelium. FGF-10 is secreted by cultured mouse pre- adipocytes, prevention of FGF-10 signaling inhibits subsequent differentiation. The ability of embryonic fibroblasts derived from FGF-10 knock-out mice to differentiate into adipocytes is also impaired.

• Description:

  Recombinant human FGF-10, (Fibroblast Growth Factor 10), is a disulfide-linked monomer protein consisting of 169 amino acids and migrates as an approximately 19 kDa protein under reducing and non-reducing conditions. Source: Optimized DNA sequence encoding human FGF-10 mature chain was expressed in E. coli.

• UniProt:

  O15520

• Endotoxin:

  < 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

• Purity:

  ≥ 96% by SDS-PAGE and HPLC

• Format:

  Lyophilized protein

• Reconstitution:

  Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

• Shipping Conditions:

  Gel Packs or Dry Ice

• Storage Conditions:

  The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

• Calculated Molecular Weight:

  19 kDa

• Formulation:

  Lyophilized from 0.2 µm filtered PBS solution, pH 7.0.