Products for Research Use Only
RegisterLogin

FAP Fluorogenic Assay Kit

CAT: 0421-80210Size: 96 ReactionsDry Ice: YesHazardous: No
Product image 1
1 / 1
CAT#:0421-80210Size:96 Reactions
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Background
Fibroblast Activation Protein, or FAP, is a type II membrane serine protease of the dipeptidyl peptidase (DPP) subfamily. It is involved in wound healing and tissue repair. FAP upregulation is seen on activated stromal fibroblasts present in cancer, including breast, lung, prostate, pancreatic and cervical cancer. It has an impact on the tumor microenvironment (TME), where it reduces the levels of PEDF (pigment epithelium derived factor), angiopoietin-1 and VEGF-c (vascular endothelial growth factor c) and increase TGF-β (transforming growth factor β) and create an immunosuppressive environment. It is used as biomarker for pro-tumorigenic stroma and links to a poor prognosis. Studies in animal models have shown that knock-out of FAP reduces the metastasis of pancreatic ductal adenocarcinomas (PDA) . It has thus been proposed as a candidate target for small molecule inhibitors for cancer therapy applications. Additionally, its expression profile makes it a good candidate for CAR-T applications, with pre-clinical and clinical trials currently ongoing.
Description
The FAP Fluorogenic Assay Kitis designed to measure FAP (fibroblast activation protein) protease activity for screening and profiling applications. It comes in a convenient 96-well format, with purified recombinant FAP enzyme (amino acids 29-760), DPP substrate, and DPP assay buffer for 100 enzyme reactions.The DPP fluorogenic substrate is incubated with a sample containing FAP enzyme to produce a fluorophore that can then be measured using a fluorescence reader.Figure 1:Illustration of the assay principle.Fluorogenic DPP Substrate 1 is a fluorogenic peptide substrate (Ala-Pro-AMC dipeptide) . In the conjugated form the energy emitted from the fluorochrome AMC is quenched. Proteolysis releases AMC and fluorescence is emitted. The increase in fluorescence is directly proportional to FAP activity.Need us to run inhibitor screens or profile your compounds against FAP? Check out ourProtease Screening Services.This product has beencited 7 times.
UniProt
Q12884
Applications
Enzyme kinetics studies and screening small molecule inhibitors for drug discovery and high-throughput screening (HTS) applications.
Format
Catalog #NameAmountStorage80100FAP, His-Tag*30 µg-80°C80300DPP Assay Buffer10 ml-20°C803050.5 mM Fluorogenic DPP Substrate 1100 μl-80°C50 µM AMC Fluorescent Standard500 μl-20°C7968596-well black microplate1Room Temp* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.
Shipping Conditions
-80°C
Storage Conditions
This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed
Notes
Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]
Contraindications
DMSO > 1%, strong acids or bases, ionic detergents, high salt

Related Products

CatalogName
78858-1PARG Fluorogenic Assay Kit
82165GPX4 Fluorogenic Assay Kit
82158ARH3 Fluorogenic Assay Kit
82154Sialidase Fluorogenic Assay Kit
78864MMP7 Fluorogenic Assay Kit
79983MMP1 Fluorogenic Assay Kit
50687HDAC11 Fluorogenic Assay Kit
50065HDAC5 Fluorogenic Assay Kit
82546ADAMTS4 Fluorogenic Assay Kit
78855Dicer Fluorogenic Assay Kit

Alternative Products

CatalogName
GWB-PS1C2AHDAC4 Fluorogenic Assay Kit
GWB-PSF8DDHDAC1 Fluorogenic Assay Kit
GWB-PS5DD4HDAC5 Fluorogenic Assay kit
GWB-PSFEF5HDAC3 Fluorogenic Assay kit
GWB-PS7C2FHDAC6 Fluorogenic Assay kit
GWB-PS1799HDAC7 Fluorogenic Assay Kit
GWB-4C8F93HDAC Fluorogenic Assay Kit
GWB-2FFDA0Fluorogenic GCN5 Assay Kit
GWB-PS3366HDAC2 Fluorogenic Assay Kit
GWB-47F4E7HDAC8 Fluorogenic Assay Kit

Popular Products