Products for Research Use Only
RegisterLogin

Dicer Fluorogenic Assay Kit

CAT: 0421-78855Size: 384 ReactionsDry Ice: YesHazardous: No
Product image 1
1 / 1
CAT#:0421-78855Size:384 Reactions
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Background
Dicer (also known as endoribonuclease Dicer or helicase with RNase motif) is a ribonuclease that participates in pre-microRNA (pre-miRNA) and double stranded RNA (dsRNA) cleavage. The cleavage of pre-miRNA into mature miRNA and formation of small interfering RNA (siRNA) from dsRNA are essential for gene regulation and DNA repair. Decreased levels of Dicer can result in Age-related Macular Degeneration (AMD), the most common age-related blindness. Dicer impairment is also involved in cancer and RNA-based viral infection. The development of small molecule inhibitors or other strategies that affect Dicer activity can provide new therapeutical avenues for cancer treatment and AMD prevention.
Description
The Dicer Fluorogenic Assay Kit is a homogeneous 384-well assay designed to measure the double-stranded-RNA processing activity of Dicer for screening and profiling applications. The Dicer Fluorogenic Assay Kit contains enough purified Dicer, substrate, and assay buffer for 384 reactions.Figure 1: Illustration of the Dicer Fluorogenic Assay method.The assay relies on an RNA duplex imitating pre-miRNA, containing a conjugated fluorophore and quencher pair. Fluorescence of the dye is effectively quenched by the proximity of the quencher in the intact RNA duplex. The endoribonuclease Dicer processes the pre-miRNA into a mature 22 bp double-stranded miRNA by cleaving the 5’ end which is conjugated to the dye, releasing it from the miRNA. Separated from the quencher, the dye emits fluorescence with excitation/emission maxima of λ=647/ 665 nm. Dicer activity is proportional to the increase in fluorescence.Need us to run inhibitor screens or profile your compounds against Dicer? Check out ourMetabolic Enzyme Screening Services.
UniProt
Q9UPY3
Applications
Screen molecules that inhibit Dicer RNAse activity in drug discovery High-Throughput Screening (HTS) applications.Determine compound IC50.Perform Dicer real-time kinetics.
Format
Catalog #NameAmountStorage79083Dicer, His-Tag (Human) *45 µg-80°CPre-micro-RNA Substrate 15 µl-80°CDR-01 Buffer3 ml-20°C384-well black microplate1Room Temp* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.
Shipping Conditions
-80°C
Storage Conditions
This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Notes
Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For further questions, please email[email protected]

Related Products

CatalogName
78858-1PARG Fluorogenic Assay Kit
82165GPX4 Fluorogenic Assay Kit
82158ARH3 Fluorogenic Assay Kit
82154Sialidase Fluorogenic Assay Kit
78864MMP7 Fluorogenic Assay Kit
79983MMP1 Fluorogenic Assay Kit
50687HDAC11 Fluorogenic Assay Kit
50065HDAC5 Fluorogenic Assay Kit
82546ADAMTS4 Fluorogenic Assay Kit
78830MMP26 Fluorogenic Assay Kit

Alternative Products

CatalogName
GWB-PS1C2AHDAC4 Fluorogenic Assay Kit
GWB-PSF8DDHDAC1 Fluorogenic Assay Kit
GWB-PS5DD4HDAC5 Fluorogenic Assay kit
GWB-PSFEF5HDAC3 Fluorogenic Assay kit
GWB-PS1799HDAC7 Fluorogenic Assay Kit
GWB-PS7C2FHDAC6 Fluorogenic Assay kit
GWB-4C8F93HDAC Fluorogenic Assay Kit
GWB-2FFDA0Fluorogenic GCN5 Assay Kit
GWB-PS3366HDAC2 Fluorogenic Assay Kit
GWB-47F4E7HDAC8 Fluorogenic Assay Kit

Popular Products