Sialidase Fluorogenic Assay Kit

CAT: 0421-82154Size: 96 ReactionsDry Ice: YesHazardous: No
CAT#:0421-82154Size:96 Reactions
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Background
Sialidases are glycosyl hydrolases that release α-2,3-, α-2,6- and α-2,8-glycosidic linkages of terminal sialic acid residues from oligosaccharides, glycoproteins, and glycolipids. TheSalmonella typhimuriumsialidase NANH has been shown to have a highKMvalue (mM range) against polyvalent targets and plays a role in enhancement of NK cell-mediated antibody-dependent cellular cytotoxicity.Salmonella typhimuriumsialidase is thought to be an immune-modulatory enzyme, therefore, its regulation may be important for therapy of diseases associated with disorders of the immune system.
Description
The Sialidase Fluorogenic Assay Kitis designed to measure Sialidase NANH (N-acylneuraminate glycohydrolase) activity for screening and profiling applications. It comes in a convenient 96-well format, with purified recombinantSalmonella typhimuriumsialidase, Sialidase substrate, Sialidase assay buffer, and Stop Solution for 100 enzyme reactions. Sialidase inhibitor DANA (N-Acetyl-2,3-dehydro-2-deoxyneuraminic acid) is also included as a control.The Sialidase fluorogenic substrate is incubated with Sialidase. Hydrolyzation of the Sialidase substrate releases a fluorophore (λexc/λem = 365 nm/445 nm) . The increase in fluorescence at λ=445 nm is directly proportional to Sialidase activity.Need us to run inhibitor screens or profile your compounds against Sialidase? Check out ourMetabolic Enzyme Screening Services.
UniProt
P29768
Applications
Enzyme kinetics studies and screening small molecule inhibitors for drug discovery and high-throughput screening (HTS) applications.
Format
Catalog #NameAmountStorage101734Sialidase, His-Tag*2 µg-80°C82795Sialidase Assay Buffer5 ml-20°C8279625 mM Sialidase Substrate10 µl-20°C827975x Stop Solution3 ml-20°C82798DANA300 μg-20°C7968596-well black microplate1Room Temp* The concentration of protein is lot-specific and will be indicated on the tube containing the protein
Shipping Conditions
-80°C
Storage Conditions
This assay kit will perform optimally for up to6 monthsfrom date of receipt when the materials are stored as directed.
Notes
Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]
Contraindications
The final concentration of DMSO in the assay should not exceed 1%.Fluorescent compounds that have λex=355-375 nm and detection at λem=435-455 nm can interfere with the readouts.It is recommended that the compound alone is tested to determine any potential interference of the compound on the assay results.

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