Sialidase Fluorogenic Assay Kit

• Background:

  Sialidases are glycosyl hydrolases that release α-2,3-, α-2,6- and α-2,8-glycosidic linkages of terminal sialic acid residues from oligosaccharides, glycoproteins, and glycolipids. TheSalmonella typhimuriumsialidase NANH has been shown to have a highKMvalue (mM range) against polyvalent targets and plays a role in enhancement of NK cell-mediated antibody-dependent cellular cytotoxicity.Salmonella typhimuriumsialidase is thought to be an immune-modulatory enzyme, therefore, its regulation may be important for therapy of diseases associated with disorders of the immune system.

• Description:

  The Sialidase Fluorogenic Assay Kitis designed to measure Sialidase NANH (N-acylneuraminate glycohydrolase) activity for screening and profiling applications. It comes in a convenient 96-well format, with purified recombinantSalmonella typhimuriumsialidase, Sialidase substrate, Sialidase assay buffer, and Stop Solution for 100 enzyme reactions. Sialidase inhibitor DANA (N-Acetyl-2,3-dehydro-2-deoxyneuraminic acid) is also included as a control.The Sialidase fluorogenic substrate is incubated with Sialidase. Hydrolyzation of the Sialidase substrate releases a fluorophore (λexc/λem = 365 nm/445 nm) . The increase in fluorescence at λ=445 nm is directly proportional to Sialidase activity.Need us to run inhibitor screens or profile your compounds against Sialidase? Check out ourMetabolic Enzyme Screening Services.

• UniProt:

  P29768

• Applications:

  Enzyme kinetics studies and screening small molecule inhibitors for drug discovery and high-throughput screening (HTS) applications.

• Format:

  Catalog #NameAmountStorage101734Sialidase, His-Tag*2 µg-80°C82795Sialidase Assay Buffer5 ml-20°C8279625 mM Sialidase Substrate10 µl-20°C827975x Stop Solution3 ml-20°C82798DANA300 μg-20°C7968596-well black microplate1Room Temp* The concentration of protein is lot-specific and will be indicated on the tube containing the protein

• Shipping Conditions:

  -80°C

• Storage Conditions:

  This assay kit will perform optimally for up to6 monthsfrom date of receipt when the materials are stored as directed.

• Notes:

  Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]

• Contraindications:

  The final concentration of DMSO in the assay should not exceed 1%.Fluorescent compounds that have λex=355-375 nm and detection at λem=435-455 nm can interfere with the readouts.It is recommended that the compound alone is tested to determine any potential interference of the compound on the assay results.