DPP8 Assay Kit

• Background:

  Dipeptidyl peptidase-8 (DPP8) is a ubiquitously expressed serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. DPP8 shares homology and substrate recognition patterns with DPP4 and like DPP4, is thought to play a role in T-cell activation and immune function.

• Description:

  The Fluorogenic DPP8 Assay Kit is designed to measure DPP8 activity using purified DPP8 for screening and profiling applications. The key to the Fluorogenic DPP8 Assay Kit is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for DPP8 reactions. The fluorometric substrate is incubated with a sample containing DPP8 enzyme to produce a fluorophore that can then be measured using a fluorescence reader.Need us to run inhibitor screens or profile your compounds against DPP8? Check out ourProtease Screening Services.This product has beencited 8 times.

• UniProt:

  Q6V1X1

• Applications:

  Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

• Format:

  CatalogNumberComponentAmountStorage80080DPP8 human recombinant enzyme5 µg-80°CAvoidfreeze/thawcycles80300DPP assay buffer10 ml-20°C80305Fluorogenic DPP substrate 1 in DMSO (0.5mM) 100 µl-80°CAMC Fluorescent standard (50 µM) 500 µl-20°C79685black, low binding NUNC black microtiter plate1 plateRoom Temp.

• Shipping Conditions:

  -80°C

• Storage Conditions:

  At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

• Notes:

  The AMC standard is included so the researcher can quatitatively determine the specific activity of the enzyme using the AMC standard as a measure of how much AMC substrate was cleaved to release free AMC.

• Contraindications:

  DMSO > 1%, strong acids or bases, ionic detergents, high salt