DPP9 Assay Kit

• Description:

  Dipeptidyl peptidase-9 (DPP9) is a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. DPP9 exhibits similar activity to that of DPP4 but unlike DPP4, DPP9 is not membrane bound.  DPP9 activity has been linked to several diseases, including type-2 diabetes, obesity and cancer. TheFluorogenic DPP9 Assay Kitis designed to measure DPP9 activity using purified DPP9 for screening and profiling applications. Using this kit, only one simple step on a microtiter plate is required for DPP9 reactions. The fluorogenic substrate is incubated with a sample containing DPP9 enzyme to produce a fluorophore that can then be measured using a fluorescence reader.Need us to run inhibitor screens or profile your compounds against DPP9? Check out ourProtease Screening Services.This product has beencited 7 times.

• UniProt:

  Q86TI2

• Applications:

  Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

• Format:

  CatalogNumberComponentAmountStorage80090DPP9 human recombinant enzyme10 µg-80°CAvoidfreeze/thawcycles!80300DPP assay buffer10 ml-20°C80305Fluorogenic DPP substrate 1 in DMSO (0.5 mM) 100 µl-80°CAMC Fluorescent standard (50 µM) 500 µl-20°C79685black, low binding NUNC blackmicrotiter plate1 plateRoomtemp.

• Shipping Conditions:

  -80°C

• Storage Conditions:

  At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

• Notes:

  The AMC standard is included so the researcher can quatitatively determine the specific activity of the enzyme using the AMC standard as a measure of how much AMC substrate was cleaved to release free AMC.

• Contraindications:

  DMSO > 1%, strong acids or bases, ionic detergents, high salt