DPP3 Assay Kit

• Description :

  The Fluorogenic DPP3 Assay Kit is designed to measure DPP3 activity using purified DPP3 for screening or profiling applications. The key to the Fluorogenic DPP3 Assay Kit is the specific, fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for DPP3 reactions; no time-consuming washing steps are required. The fluorometric substrate is incubated with a sample containing DPP3 enzyme to produce a fluorophore that can then be measured using a fluorescence reader.Need us to run inhibitor screens or profile your compounds against DPP3? Check out ourProtease Screening Services.This product has beencited 2 times.

• UniProt :

  Q9NY33

• Applications :

  Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

• Format :

  Catalog #ComponentAmountStorage80030DPP3 human recombinant enzyme*5 µg-80°CAvoid freeze/ thaw cycles80300DPP assay buffer10 ml-20°C80332Fluorogenic DPP substrate 2 in DMSO (0.5 mM) 100 µl-80°CFluorescent AMC standard (50 µM) 500 µl-20°C79685black, low binding NUNC blackmicrotiter plate1 plateRoom Temp*The concentration of DPP3 is lot-specific and will be indicated on the tube containing the enzyme

• Shipping Conditions :

  -80°C

• Storage Conditions :

  At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

• Notes :

  The AMC standard is included so the researcher can quatitatively determine the specific activity of the enzyme using the AMC standard as a measure of how much AMC substrate was cleaved to release free AMC.

• Contraindications :

  DMSO > 1%, strong acids or bases, ionic detergents, high salt