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IDO1 Fluorogenic Inhibitor Screening Assay Kit -384

CAT: 0421-72047Size: 384 WellsDry Ice: YesHazardous: No
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CAT#:0421-72047Size:384 Wells
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Background
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.
Description
TheIDO1 Fluorogenic Inhibitor Screening Assay Kitis designed to measure enzyme inhibition of indoleamine 2,3-dioxygenase 1 (IDO1) . TheIDO1 Fluorogenic Inhibitor Screening Assay Kitis simple to use and detects fluorescence at long wavelengths, which minimizes potential errors due to compound interference. In the assay, the inhibitor and enzyme are added to a sample containing L-Trp substrate. After a 1 hour incubation at room temperature, the fluorescence solution is added and incubated at 37°C for four hours. Activity is measured by reading sample fluorescence at λ=510 nm following excitation of the reaction product at λ=400 nm.
UniProt
P14902
Applications
Useful for the study of IDO1 enzymology, inhibitor screening, and selectivity profiling.
Format
CatalogNumberComponentAmountStorage71182IDO1 His-Tag2 x 80 µg-80°CAvoid multiple freeze/ thaw cycles!73009IDO1 Fluorogenic Reaction Solution3 x 10 ml-80°C73002IDO1 Assay Buffer5 ml-80°CFluorescence Solution10 ml-80°CBlack 384 Well Assay-Plate1Plate Sealing Film1
Shipping Conditions
-80°C (Dry Ice)
Storage Conditions
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Notes
License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. Publications using this cell line should reference BPS Bioscience, Inc., San Diego
Contraindications
DMSO > 0.5%, strong acids or bases, ionic detergents, high salt. Warning: the Fluorescence Solution contains a component that is known to be a skin and eye irritant. Use caution and appropriate personal protective equipment when handling this component.

UniProtKB · P14902

Indoleamine 2,3-dioxygenase 1

I23O1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P14902
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IDO1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
IDO-1
EC number
1.13.11.52
Processing
—
Secondary accessions
E5RGR8, F6M9T7, Q540B4
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Cellular component

Cytoplasm

Molecular function

DioxygenaseOxidoreductase

Ligand

HemeIronMetal-binding

Biological process

ImmunityTryptophan catabolism

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