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IDO1 Fluorogenic Inhibitor Screening Assay Kit

CAT: 0421-72037Size: 96 ReactionsDry Ice: YesHazardous: No
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CAT#:0421-72037Size:96 Reactions
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.
Description
TheIDO1Fluorogenic Inhibitor Screening Assay Kitis designed to measure enzyme inhibition of indoleamine 2,3-dioxygenase 1 (IDO1) . TheIDO1 Fluorogenic Inhibitor Screening Assay Kitis simple to use and detects fluorescence at long wavelengths, which minimizes potential errors due to compound interference. In the assay, the inhibitor and enzyme are added to a sample containing L-Trp substrate. After a 1 hourincubation at room temperature, the fluorescence solution is added and incubated at 37oC for four hours. Activity is measured by reading sample fluorescence at l=510 nm following excitation of the reaction product at l=400 nm.
UniProt
P14902
Applications
Useful for the study of IDO1 enzymology, inhibitor screening, and selectivity profiling.
Format
CatalogNumberComponentAmountStorage71182IDO1 His-Tag40 μg-80°CAvoidmultiplefreeze/thawcycles!73009IDO1 Fluorogenic Reaction Solution2 x 10 ml-80°C73002IDO1 Assay Buffer1 ml-80°CFluorescence Solution2 x 1 ml-80°C79685Black 96 Well Assay-Plate1RTPlate Sealing Film1
Shipping Conditions
-80°C (Dry Ice)
Storage Conditions
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival
Contraindications
DMSO > 0.5%, strong acids or bases, ionic detergents, high salt. Warning: the Fluorescence Solution contains a component that is known to be a skin and eye irritant, use caution and appropriate personal protective equipment when handling this component.

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