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TDO2 Fluorogenic Inhibitor Screening Assay Kit (Human)

CAT: 0421-72039Size: 96 ReactionsDry Ice: YesHazardous: No
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CAT#:0421-72039Size:96 Reactions
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. TDO (Tryptophan 2,3 dioxygenase) is a heme-containing protein, involved in the catabolism of L-Trp to Kyn. Its activity results in the consumption of L-Trp and an accumulation of Kyn. This metabolism maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Accumulation of Kyn also has an impact on neurons and brain related diseases. TDO is upregulated in many tumors, providing cancer cells with an avenue for immune evasion. The use of TDO inhibitors may be a viable therapeutic strategy in cancer therapy and neuroscience related disorders.
Description
The TDO2 Fluorogenic Inhibitor Screening Assay Kit (Human) is designed to measure TDO2 (tryptophan 2,3-dioxygenase 2) activity for screening and profiling applications. The assay kit comes in a convenient 96-well format, with enough recombinant TDO2, fluorogenic substrate and solution, and assay buffer for 100 enzyme reactions.Figure 1: Illustration of the mechanism behind the TDO2 Fluorogenic Inhibitor Screening Assay Kit (Human) .TDO2 converts the conversion of tryptophan to N-formylkynurenine (NFK), which can react with the TDO Fluorogenic reagent present in the TDO Fluorogenic Reaction Solution and generate a green fluorescent molecule. The increase in fluorescence is proportional to TDO2 activity.
UniProt
P48775
Applications
Study enzyme kinetics and screen small molecule inhibitors of TDO for drug discovery and high throughput screening (HTS) applications.
Format
Catalog #NameAmountStorage71195TDO2, His-Tag (Human) *50 µg-80°C73010TDO Fluorogenic Reaction Solution2 x 10 ml-80°C73006TDO Buffer1 ml-80°CFluorescence Solution2 x 1 ml-80°CBlack, low binding 96- microtiter plate1Room TempPlate Sealing Film1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.
Shipping Conditions
-80°C
Storage Conditions
This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Notes
Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]
Contraindications
The final concentration of DMSO in the assay should not exceed 0.5%.Compounds that are fluorescent may interfere with the results, depending on their spectral excitation and emission properties.It is recommended that the compound alone is tested to determine any potential interference of the compound on the assay results.The presence of strong acids or bases, ionic detergents and high salt should be avoided.

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