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Recombinant Vimentin Antibody

CAT: 0800-V9284-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V9284-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Anti-Vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P08670
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Cat, Cow, Horse
Immunogen
A portion of amino acids 2-466 from the human protein was used as the immunogen for the recombinant Vimentin antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
VIM/6430R
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant Vimentin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant Vimentin antibody is available for research use only.
Storage Conditions
Aliquot the recombinant Vimentin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant Vimentin antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
IHC staining of FFPE human tonsil tissue with recombinant Vimentin antibody (clone VIM/6430R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

UniProtKB · P08670

Vimentin

VIME_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P08670
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
VIM
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B0YJC2, D3DRU4, Q15867, Q15868, Q15869, Q548L2, Q6LER9, Q8N850, Q96ML2, Q9NTM3
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationGlycoproteinIsopeptide bondPhosphoproteinS-nitrosylationUbl conjugation

Disease

CataractDisease variant

Cellular component

Cell membraneCell projectionCytoplasmCytoskeletonIntermediate filamentMembraneNucleus

Domain

Coiled coil

Biological process

Host-virus interaction
Product Datasheet
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