Vimentin Antibody

CAT: 0800-V8161-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V8161-100UGSize:100 µg
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Description
This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 1-2 µg/mL
UniProt
P08670
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human partial protein (amino acids 2-466) was used as the immunogen for this Vimentin antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
VIM/3736
Applications
IHC-P, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Vimentin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Vimentin antibody is available for research use only.
Storage Conditions
Store the Vimentin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Vimentin antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
IHC staining of FFPE human colon carcinoma with Vimentin antibody (clone VIM/3736) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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