Vimentin Antibody

CAT: 0800-V8160-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V8160-100UGSize:100 µg
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Description
This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P08670
Host
Mouse
Reactivity
Human
Immunogen
Vimentin protein purified from porcine eye lens cells was used as the immunogen for this Vimentin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
V9
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Vimentin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Vimentin antibody is available for research use only.
Storage Conditions
Store the Vimentin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Vimentin antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Western blot testing of human U-87 cell lysate with Vimentin antibody (clone V9) . Predicted molecular weight ~53 kDa.

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