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PKC alpha Antibody

CAT: 0800-F50026-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
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CAT#:0800-F50026-0.08MLSize:0.08 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC alpha is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca (2+) handling in myocytes.
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50, Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50
UniProt
P17252
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 299-329 from the human protein was used as the immunogen for this PKC alpha antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC, IF, FACS, WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the PKC alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This PKC alpha antibody is available for research use only.
Storage Conditions
Aliquot the PKC alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the PKC alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse, Rabbit, Rat
Image Legend
Confocal immunofluorescent analysis of PKC alpha antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red) . DAPI was used as a nuclear counterstain (blue) .

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