Alpha-2-Macroglobulin Antibody / A2M

CAT: 0800-V8506-20UGSize: 20 µgDry Ice: NoHazardous: No

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CAT#:0800-V8506-20UGSize:20 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Alpha-2-Macroglobulin (A2M) is a homotetrameric serum protein consisting of four identical subunits that form dimers through disulfide bonds. Initially, it was characterized as a pan-proteinase inhibitor that was able to bait proteinases into cleaving specific peptide sequences on A2M. This interaction induces a conformational change, thus enabling it to trap the proteinase and further inhibit its activity. Subsequently, A2M has been shown to function as a carrier protein and regulator of cytokines during inflammation.

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P01023

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 604-748 from the human protein was used as the immunogen for the Alpha-2-Macroglobulin antibody.

Clonality

Monoclonal

Isotype

IgG2c κ

Clone

A2M/3621

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Alpha-2-Macroglobulin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Alpha-2-Macroglobulin antibody is available for research use only.

Storage Conditions

Store the Alpha-2-Macroglobulin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Alpha-2-Macroglobulin antibody should be determined by the researcher.

Location

Secreted

Image Legend

IHC staining of FFPE human liver with Alpha-2-Macroglobulin antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

UniProtKB · P01023

Alpha-2-macroglobulin

A2MG_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P01023
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: A2M
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: Alpha-2-M
EC number: —
Processing: Precursor
Secondary accessions: Q13677, Q59F47, Q5QTS0, Q68DN2, Q6PIY3, Q6PN97

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Domain

Bait regionSignal

PTM

Disulfide bondGlycoproteinIsopeptide bondThioester bond

Molecular function

Protease inhibitorSerine protease inhibitor

Cellular component