CD8b Antibody

• Description :

  This monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. This monoclonal antibody identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.

• Specifications :

  Functional Studies (order BSA/azide-free format), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL

• UniProt :

  P10300

• Host :

  Rat

• Reactivity :

  Mouse

• Immunogen :

  Five day mixed leukocyte culture population, C57BL/6 anti-BALB/c

• Clonality :

  Monoclonal

• Isotype :

  IgG2b κ

• Clone :

  H35-17.2

• Applications :

  Functional Studies, FACS, IF

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution :

  Store the CD8b antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This CD8b antibody is available for research use only.

• Storage Conditions :

  Store the CD8b antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes :

  Optimal dilution of the CD8b antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Image Legend :

  SDS-PAGE analysis of purified, BSA-free CD8b antibody (clone H35-17.2) as confirmation of integrity and purity.