CD8b Antibody

• Description:

  This monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. This monoclonal antibody identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.

• Specifications:

  Functional Studies (order BSA/azide-free format), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL

• UniProt:

  P10300

• Host:

  Rat

• Reactivity:

  Mouse

• Immunogen:

  Five day mixed leukocyte culture population, C57BL/6 anti-BALB/c

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  H35-17.2

• Applications:

  Functional Studies, FACS, IF

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the CD8b antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD8b antibody is available for research use only.

• Storage Conditions:

  Store the CD8b antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD8b antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  SDS-PAGE analysis of purified, BSA-free CD8b antibody (clone H35-17.2) as confirmation of integrity and purity.