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HER-2 Antibody / ErbB2

CAT: 0800-V7876SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V7876SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P04626
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human HER2 protein fragment within amino acids 311-462 was used as the immunogen for the HER-2 antibody. Its epitope is localized in the extracellular domain.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
ERBB2/3080
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the HER-2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This HER-2 antibody is available for research use only.
Storage Conditions
Store the HER-2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the HER-2 antibody should be determined by the researcher.
Location
Cell surface
Image Legend
IHC staining of FFPE human breast carcinoma with HER-2 antibody (clone ERBB2/3080) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

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