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Recombinant CD81 Antibody

CAT: 0800-V7501-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V7501-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1) . CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.
CAS Number
9000-83-3
Specifications
ELISA: order BSA/sodium azide-free format for coating, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 0.5-1ug/million cells
UniProt
P60033
Host
Rabbit
Reactivity
Human
Immunogen
A B-cell line derived from a Burkitt lymphoma was used as the immunogen for the recombinant CD81 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
C81/2885R
Type
Recombinant
Applications
ELISA, IHC-P, FACS
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the recombinant CD81 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant CD81 antibody is available for research use only.
Storage Conditions
Store the recombinant CD81 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic, membranous
Image Legend
Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells using recombinant CD81 antibody (clone C81/2885R) . Gray=unstained cells, Orange = recombinant CD81 antibody.
Product Datasheet
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