HSP27 Antibody

• Description:

  HSP27 is induced by environmental stress and developmental changes. It is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. [RefSeq]

• Specifications:

  Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

• UniProt:

  P04792

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human HSPB1 protein was used as the immunogen for the HSP27 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  SRPR27

• Applications:

  IF, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the HSP27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This HSP27 antibody is available for research use only.

• Storage Conditions:

  Store the HSP27 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Predominantly cytoplasmic with some nuclear

• Image Legend:

  IHC testing of FFPE human prostate carcinoma and HSP27 antibody (clone SRPR27) . Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.