HSP27 Antibody

• Description:

  This antibody recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27) . HSP27 was recently found to be identical to the estrogen-induced 'p29' and '24K' protein. About 50% of breast carcinomas are positive for HSP27, especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.

• Specifications:

  Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P04792

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Partially purified human HSP27 (earlier called 24K) from breast cancer MCF-7 cells was used as the immunogen for this antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  G3.1

• Applications:

  FACS, IF, WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the HSP27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This HSP27 antibody is available for research use only.

• Storage Conditions:

  Store the HSP27 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Predominantly cytoplasmic with some nuclear

• Image Legend:

  IHC testing of FFPE human breast carcinoma (10X) stained with HSP27 antibody (clone G3.1) .