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HSP27 Antibody

CAT: 0800-R31650Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31650Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
HSPB1 (Heat shock 27kDa protein 1), also known as HSP27, is a protein that in humans is encoded by the HSPB1 gene. The protein encoded by this gene is induced by environmental stress and developmental changes. HSP27 is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN) .
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
Host
Rabbit
Reactivity
Human
Immunogen
Human partial recombinant protein (AA 1-205) was used as the immunogen for this HSP27 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the HSP27 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This HSP27 antibody is available for research use only.
Storage Conditions
After reconstitution, the HSP27 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the HSP27 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Immunofluorescent staining of FFPE human U-2 OS cells with HSP27 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.
Product Datasheet
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