HSP27 Antibody

• Background:

  In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSBP1 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g. phorbol esters) and calcium ionophores, and high levels are associated with carcinoma of the breast and with endometrial adenocarcinomas. Heat shock of HeLa cell cultures, or treatment with arsenite, phorbol ester, or tumor necrosis factor, causes a rapid phosphorylation of preexisting HSBP1, with Ser82 as the major site and Ser78 the minor site of phosphorylation. HSBP1 may exert phosphorylation-activated functions linked with growth signaling pathways in unstressed cells. A homeostatic function at this level could protect cells from adverse effects of signal transduction systems which may be activated inappropriately during stress.

• NCBI Gene ID:

  3315

• Swiss Prot:

  P04792

• Accession Number:

  P04792

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This HSP27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-136 amino acids from the Central region of human HSP27.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle, Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  23 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  HSPB1

• NCBI Official Name:

  Heat shock protein beta-1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Wano, C., et al., Exp. Cell Res. 298 (2) :584-592 (2004) .

• Background Reference 02:

  Evgrafov, O.V., et al., Nat. Genet. 36 (6) :602-606 (2004) .

• Background Reference 03:

  Song, H., et al., Biochem. Biophys. Res. Commun. 314 (1) :143-150 (2004) .

• Background Reference 04:

  Chauhan, D., et al., Blood 102 (9) :3379-3386 (2003) .

• Other Product Names:

  Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27, HSPB1, HSP27, HSP28

• Tested Applications:

  WB, IHC-P

• Protein ID:

  19855073

• Physical Properties:

  Liquid