Products for Research Use Only

HSP27 Antibody

CAT: 0800-V2134IHC-7MLSize: 7 mLDry Ice: NoHazardous: No
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CAT#:0800-V2134IHC-7MLSize:7 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27) . HSP27 was recently found to be identical to the estrogen-induced 'p29' and '24K' protein. About 50% of breast carcinomas are positive for HSP27, especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
CAS Number
9007-83-4
Specifications
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
UniProt
P04792
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Partially purified human HSP27 (earlier called 24K) from breast cancer MCF-7 cells was used as the immunogen for this antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
G3.1
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the HSP27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This HSP27 antibody is available for research use only.
Storage Conditions
Store the HSP27 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Location
Predominantly cytoplasmic with some nuclear
Image Legend
IHC testing of FFPE human breast carcinoma (10X) stained with HSP27 antibody (clone G3.1) .

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