CD20 Antibody

• Description:

  CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. It is expressed by pre B-cells and persists during all stages of maturation but is lost upon terminal differentiation into plasma cells. The protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. It acts as a calcium channel involved in B-cell activation and cell cycle progression.

• Specifications:

  FACS: 0.5-1ug/million cells, Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

• UniProt:

  P11836

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant CD20 protein was used as the immunogen for this CD20 antibody. The epitope is known to be extracellular.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  CDLA20-1

• Applications:

  FACS, IF, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  CD20 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

• Limitations:

  This CD20 antibody is available for research use only.

• Storage Conditions:

  CD20 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Predominantly cell surface with some cytoplasmic

• Image Legend:

  Immunofluorescent staining of human Raji cells with CD20 antibody (clone CDLA20-1, green) and Reddot nuclear stain (red) .