CD28 Antibody

• Description:

  Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

• Specifications:

  Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-3 µg/mL

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human protein was used as the immunogen for this CD28 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  CB28

• Applications:

  FACS, IF

• Purity:

  Protein G affinity chromatography

• Format:

  CF488 Conjugate

• Buffer:

  500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the CD28 antibody at 2-8oC, protected from light.

• Limitations:

  This CD28 antibody is available for research use only.

• Storage Conditions:

  Store the CD28 antibody at 2-8°C, protected from light.

• Formulation:

  500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD28 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Flow cytometry staining of PFA-fixed human Jukat cells with unlabeled CD28 antibody (clone CB28) ; Red=isotype control, Blue= CD28 antibody.