Emerin Antibody

• Description:

  Emerin is a member of the nuclear lamina associated protein family. It is ubiquitously expressed and localized to the nuclear membrane in normal cells. Mutations of the gene that encodes emerin result in the X-linked recessive disease Emery-Dreifuss muscular dystrophy (EDMD), which is characterized by slowly progressing contractures, skeletal muscle wasting and cardiomyopathy. Reportedly, lack of Emerin expression is one cause of EDMD. Emerin is involved in the association of the nuclear membrane with the lamina, and is localized specifically to desmosomes and fasciae adherents in the heart. Identification of nuclear membrane irregularities with anti-emerin antibody has been reported useful in diagnosing papillary thyroid carcinoma.

• Specifications:

  Western blot: 0.5-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P50402

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 56-167 from the human protein was used as the immunogen for the Emerin antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  EMD/2168

• Applications:

  WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the Emerin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Emerin antibody is available for research use only.

• Storage Conditions:

  Store the Emerin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Titering of the Emerin antibody may be required for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Nuclear membrane

• Image Legend:

  IHC testing of FFPE human basal cell carcinoma with Emerin antibody (clone EMD/2168) . Required HIER: boiling tissue sections in 10mM citrate buffer, pH 6, for 10-20 min and allow to cool prior to staining.