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Emerin Antibody

CAT: 0800-RQ4516Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4516Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta- catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C. [UniProt]
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P50402
Host
Mouse
Reactivity
Human
Immunogen
Amino acids MDNYADLSDTELTTLLRRYNIPHGPVVGSTRRLYEKKIFEYETQRRRL were used as the immunogen for the Emerin antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
5A10
Applications
WB, IHC-P
Purity
Protein G affinity
Format
Purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the Emerin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Emerin antibody is available for research use only.
Storage Conditions
After reconstitution, the Emerin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Emerin antibody should be determined by the researcher.
Location
Nuclear membrane
Image Legend
Western blot testing of human 1) HeLa, 2) placenta, 3) Caco-2, 4) HepG2, 5) Rabbit IgG, 6) marker, 7) Jurkat, 8) MDA-MB-453, 9) SK-OV-3 and 10) U-87 MG lysate with Emerin antibody at 0.5ug/ml. Expected molecular weight: 29-34 kDa.

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